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Bacterial Conjugation Experiment
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INTRODUCTION: Bacteria, in general, reproduce asexually, but in order to increase diversity, they have developed a mechanism for transfer of genetic material from one bacterium to another. The ability to perform this transfer is conferred by a set of genes which .... Middle of Essay
.... can be measured in minutes from the origin of transfer. That is, the amount of time it takes for a particular gene to be transferred from one bacterium to another indicated how far it is from the origin of replication.
METHODS AND MATERIALS: Media Preparation: The starting material was Medium 56-glucose agar (MM560). The components of the MM56 are found in figure 1. From this, we made two types of media, complete and selective. The reagents used, along with their stock and final concentrations are found in figure 2. The formula:
Stock volume = [(Final concentration)(final volume)] / (stock concentration)
was used to calculate the amounts of each r ....
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Words: 1154 - Pages: 5
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