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Chromatography

Beginning of Essay
is a separation technique in which the mixture to be separated is dissolved in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the original mixture depen ....

Middle of Essay
.... liquid consists of a column with a fritted bottom that holds a stationary phase in equilibrium with a solvent. Typical stationary phases (and their interactions with solutes) are: solids (adsorption), ionic groups on a resin (ion-exchange), liquids on an inert solid support (partitioning), and porous inert particles (size exclusion). The mixture to be separated is loaded onto the top of the column followed by more solvent. The different components in the sample mixture pass through the column at different rates due to differences in their partition behavior between the mobile phase and the stationary phase. The compounds are separated b ....

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