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Green Fluorescent Protein Purification
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Green Fluorescent Protein Purification
Green Fluorescent Protein (GFP) is a relatively new biochemical tool with a wide range of applications in current research. The natural function of GFP is to facilitate the bioluminescence of jellyfish and other coelenterate .... Middle of Essay
.... So GFP is basically a stable little (27-30 kD) green light bulb that can be tacked on to other proteins at either the N- or C-terminus without a distortion of its compact beta-can structure. Biochemists now frequently exploit GFP’s unique structural and physical characteristics to visualize various cellular activities.
One such application of GFP is to understand the role of a hormone, T3, in the translocation of thyroid hormone nuclear receptors (TRs).* In this project, GFP was fused to the human TR subtype b1 protein and the protein’s intracellular movement was visually tracked in live cells in the presence or absence of the T3 hormone. ....
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Words: 1280 - Pages: 5
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