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Determination Of An Unknown Amino Acid From Titration
Beginning of Essay
Abstract
Experiment 11 used a titration curve to determine the identity of an
unknown amino acid. The initial pH of the solution was 1.96, and the pKa’s
found experimentally were 2.0, 4.0, and 9.85. The accepted pKa values were
found to be 2.10, 4.07, and 9.4 .... Middle of Essay .... group is a
Lewis acidic because it is able to donate a hydrogen ion (Kotz et al., 1996).
Other forms of amino acids also exist. Amino acids may exists as acidic or
basic salts. For example, if the glycine reacted with HCl, the resulting amino
acid would be glycine hydrochloride (see fig. 2). Glycine hydrochloride is an
example of an acidic salt form of the amino acid. Likewise, if NaOH were added,
the resulting amino acid would be sodium glycinate (see fig. 3), an example of a
basic salt form.
Fig. 2
Fig. 3
Due to the nature of amino acids, a titration curve can be employed to identify
an unknown amino acid. A titration curve is the plo ....
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