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PCR And Its Use
Beginning of Essay
Often times, scientists only have a small amount of DNA to deal with when doing
genetic research or studies. In these situations, scientists can do one of
several things. One is to just try to work with it anyway, but this is nearly
impossible (depending on how .... Middle of Essay
.... etc). Then they have
to run it through the PCR machine. Here is a (rather detailed) description of
the process: "The cycling protocol consisted of 25-30 cycles of three-
temperatures: strand denaturation at 95degC, primer annealing at 55degC, and
primer extension at 72deg C, typically 30 seconds, 30 seconds, and 60 seconds
for the DNA Thermal Cycler and 4 seconds, 10 seconds, and 60 seconds for the
Thermal Cycler 9600, respectively."
Basically, that means that they set it to certain temperatures, then put it in
different cyles for different amounts of time. PCR machines can be compared
with washing machines. There are the different temperatu ....
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Words: 773 - Pages: 3
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