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Bacteria Classification By Gram Staining
Beginning of Essay
THE AMERICAN UNIVERSITY IN CAIRO
BIOLOGY DEPARTMENT
SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1
Objective:
To test a sample of laboratory prepared bacteria and categorise it
according to Christian’s gram positive and gram negative classes and also by
vie .... Middle of Essay
.... Alcohol d.
Safranin e. Distilled Water 4. Bibulous Blotting Paper 5. Microscope 6. Oil
Procedure:
A. Preparation :
1. Bacteria is cultivated on agar jelly in an incubator at 25°C for 24 hours. 2.
Obtain a microscope slide and with a toothpick, smear a thin coat of the
bacteria sample onto the slide 3. Cover the smear with a drop Crystal Violet
and leave standing for 20 seconds 4. Wash off the stain with distilled water;
drain and blot off the excess with bibulous paper. 5. Apply Gram’s Iodine on
the smear and leave to stand for 1 minute. 6. Drain the excess iodine and apply
95% Ethyl alcohol for 20 second duration or till the alco ....
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Words: 751 - Pages: 3
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